☰ Contents · Biology

The hand lens and the microscope

Lessons 5 · 1 lessons · O‘. Pratov, A. To‘xtayev, F. Azimova, Z. Tillayeva. Biology, Grade 5. Revised and expanded 5th edition. “O‘zbekiston” publishing house, Tashkent, 2020
5

Lab 2: The hand lens and the microscope

Textbook: pp. 15–17
GoalKnow the parts of a hand lens and a light microscope, calculate microscope magnification and state the steps for using it.
New words
hand lens (magnifying glass) · lupamicroscope · mikroskopeyepiece lens · okulyarobjective lens · obyektiv
Explanation

To study the structure of living things we need magnifying instruments. The simplest is the hand lens: a lens bulging on both sides that magnifies an object about 2–20 times. With it you can notice the round cells in the flesh of a watermelon or tomato. A microscope is a laboratory instrument that shows even smaller objects. Its stand carries the tube and the stage. At the top of the tube is the eyepiece (ocular) and at the bottom the objective; both are magnifying lenses. The coarse-focus screw raises and lowers the tube, the fine-focus screw makes the image sharp. Under the stage is a mirror that directs light onto the specimen (modern microscopes often have a built-in lamp), and a clip holds the specimen in place. The magnification of a microscope equals the eyepiece number times the objective number: eyepiece 10, objective 20 gives 10 × 20 = 200 times. The specimen is viewed first with the small objective and then with the large one, the microscope is not moved while you work, and the image is drawn in the notebook.

Worked examples
With eyepiece 10 and objective 40, the microscope magnifies the object 10 × 40 = 400 times.
To keep the objective from hitting the slide, we first lower the tube, watching from the side, until the objective is about 4–5 mm above the specimen, then look through the eyepiece and slowly raise the tube.
Class activity

Teacher-led: label the nine parts on a picture of the microscope (eyepiece, coarse screw, fine screw, stand, stage, mirror, clip, objective, tube). Then, with the teacher, look at a ready-made specimen with the small objective and draw it. Safety: the microscope is taken only with the teacher’s permission and carried with two hands; never point the mirror straight at the sun, which can damage the eyes; do not touch the lenses with your fingers.

Practice
1
About how many times does a hand lens magnify an object?
2
How many times does a microscope with eyepiece 10 and objective 40 magnify?
3
Which part of the microscope raises and lowers the tube, and which makes the image sharp?
4
Why do we look at the specimen first with the small objective and then the large one?