Lessons 13–14 · 2 lessons · A. G‘afurov, A. Abdukarimov, J. Tolipova, O. Ishankulov, M. Umaraliyeva, I. Abdurahmonova. Biology Grade 11, 1st edition. Sharq Publishing and Printing Joint-Stock Company, Tashkent, 2018
14
Laboratory work 1
Textbook: pp. 79–80
GoalStudy the effect of abiotic factors (light, moisture) on plant growth with a controlled experiment, record data in a table and draw conclusions.
New words
control group · nazorat guruhivariable · o‘zgaruvchi (omil)replication · takrorlanishconclusion · xulosa
Explanation
A laboratory experiment finds a causal link by changing one factor while keeping the others equal. It has an independent variable (for example light or the watering rate), a dependent variable (plant height, leaf count, colour) and a control group (normal conditions). Each variant uses several plants (replication), because the chance result of a single plant is unreliable. Measurements are taken at the same time and in the same way, results are put into a table, and the mean is calculated. For example, bean or wheat seeds can be grown in four cups: (1) light + normal watering (control), (2) light + little water, (3) dark box + normal watering, (4) dark + little water. Seedlings grown in the dark are pale, long and thin (etiolation) because chlorophyll does not form and the plant stretches toward light. The conclusion rests on the results and applies only to the factors studied.
Worked examples
Four bean seedlings in the lit cup measured 8, 10, 12 and 10 cm after 2 weeks. Mean height = (8 + 10 + 12 + 10) : 4 = 10 cm. Those grown in the dark measured 14, 18, 16, 20 cm: mean 17 cm, but they are pale and thin – a greater height does not mean a healthy plant.
Of 24 seeds, 21 germinated in the control dish and 15 in the under-watered dish. Germination: control 21·100 : 24 = 87.5 %; under-watered 15·100 : 24 = 62.5 %. The difference suggests that lack of moisture reduces germination (to be confirmed by repeating the experiment).
Class activity
Observe the experiment started a week earlier: measure two seedlings per cup with a ruler, note the colour, calculate the mean. Use only clean water and ordinary soil; do not put seedlings in your mouth, and wash hands afterwards. Do not use chemical fertilisers or pesticides.
Practice
1
Why is a control group needed in an experiment?
To compare results with normal conditions and identify the effect of the changed factor.
2
Seedling heights are 6, 9, 9 and 12 cm. What is the mean height in cm?
9
3
Of 40 seeds, 34 germinated. What is the germination percentage?
85
4
Why is a seedling grown in the dark pale and elongated?
Without light chlorophyll does not form; the plant stretches its stem searching for light (etiolation).