☰ Contents · Biology (Cytology and genetics)

Dihybrid problems, herbarium and gene interaction

Lessons 51–53 · 3 lessons · A. Zikiryayev, A. To‘xtayev, I. Azimov, N. Sonin. Biology. Basics of Cytology and Genetics, Grade 9, revised and expanded 5th edition. “Yangiyul Poligraph Service”, Tashkent, 2019
51

Practical work 1: solving dihybrid cross problems

Textbook: p. 141
GoalSolve dihybrid cross problems: write gametes, calculate ratios, find parents’ genotypes.
New words
dihybrid heterozygote · digeterozigotagamete types · gameta turlariphenotype class · fenotipik sinfoffspring ratio · avlod nisbati
Explanation

In a dihybrid problem first write the gametes of each organism: every gamete carries one gene of each allele pair (AaBb — AB, Ab, aB, ab; AABb — AB, Ab; aabb — only ab). The number of gamete types is 2ⁿ, where n is the number of heterozygous pairs. Without building a big Punnett square you can work out each trait separately and multiply the ratios, because the traits are inherited independently: Aa × Aa gives 3:1 and Bb × bb gives 1:1, so AaBb × Aabb gives (3:1)(1:1) = 3:3:1:1. To find the parents’ genotypes, split the ratio in F by trait: 3:1 — both parents heterozygous (Aa × Aa), 1:1 — Aa × aa, all dominant and uniform — at least one parent is homozygous dominant (AA). For counting, divide by the sum of the ratio parts and multiply by each class.

Worked examples
AaBb × aaBb: trait A is Aa × aa = 1:1, trait B is Bb × Bb = 3:1; together (1:1)(3:1) = 3:1:3:1. Of 800 offspring A_B_ = 800 ÷ 8 × 3 = 300.
Tomato: round (A) is dominant over pear-shaped (a), red (B) over yellow (b). AaBb × aabb gives four phenotypes 1:1:1:1: round-red, round-yellow, pear-red, pear-yellow.
Class activity

“Gamete race”. 8 genotypes are written on the board (AABB, AaBB, aaBb, AaBb, Aabb, aabb, AABb, aaBB); groups quickly write each one’s gametes and their number.

Practice
1
What gametes does an AaBB organism produce?
2
What is the phenotype ratio in the offspring of AaBb × Aabb?
3
How many kinds of gametes does AaBbCcDd form?
4
Why can ratios be worked out per trait and multiplied?