☰ Contents · Biology (Cytology and genetics)

Observing cells (laboratory works 2 and 3)

Lessons 17–18 · 2 lessons · A. Zikiryayev, A. To‘xtayev, I. Azimov, N. Sonin. Biology. Basics of Cytology and Genetics, Grade 9, revised and expanded 5th edition. “Yangiyul Poligraph Service”, Tashkent, 2019
17

Laboratory work 2: studying plant and animal cells under the microscope

Textbook: p. 51
GoalObserve onion skin and cheek lining cells under the microscope and compare plant and animal cells.
New words
epidermis · epidermisiodine solution · yod eritmasicell wall · hujayra devorimucous lining cells · shilliq qavat hujayralari
Explanation

The work is done under the teacher’s guidance. Place a thin piece of red onion skin (peeling the layer off is shown by the teacher or an adult) in a drop of water on a slide, cover it with a cover slip and view it first on low and then on high power. You see polygonal cells, a thick wall, a coloured vacuole and a nucleus. For cheek cells use only your own clean, single-use stick or cotton swab to lightly rub the inside of the cheek and smear the sample into a drop of iodine solution on a slide; the iodine solution (supplied by the teacher) must not get into the mouth, and must not be swallowed. An animal cell is flat, of irregular shape, with no wall, and a granular cytoplasm and nucleus are visible. Afterwards dispose of used material as the teacher instructs and wash your hands with soap; never work with another student’s sample.

Worked examples
Onion cell: clear wall, polygonal shape, coloured vacuole. Cheek cell: no wall, flat shape, the nucleus stains yellow-brown with iodine.
A drawing label: “1 – cell wall, 2 – cytoplasm, 3 – nucleus, 4 – vacuole”. The magnification is stated (e.g. 10 × 40 = 400×).
Class activity

“Detective”. The teacher shows two pictures (onion and cheek cells); groups prove which is the plant cell using at least three features.

Practice
1
Which parts are visible in onion cells under the microscope?
2
State two visible differences between plant and animal cells.
3
Calculate the magnification of a cell viewed with a 10× eyepiece and a 40× objective.
4
Why do we stain cheek cells with iodine and not use another person’s sample?