☰ Contents · Biology (Cytology and genetics)

Chemosynthesis and protein biosynthesis

Lessons 34–35 · 2 lessons · A. Zikiryayev, A. To‘xtayev, I. Azimov, N. Sonin. Biology. Basics of Cytology and Genetics, Grade 9, revised and expanded 5th edition. “Yangiyul Poligraph Service”, Tashkent, 2019
35

Plastic metabolism in the cell

Textbook: pp. 88–92
GoalExplain DNA replication, transcription, the genetic code and translation (protein biosynthesis) step by step and solve simple problems.
New words
replication · replikatsiyatranscription · transkripsiyatranslation · translyatsiyagenetic code (codon) · genetik kod (kodon)
Explanation

In replication enzymes separate the strands of the DNA double helix and build a new complementary strand on each, producing two identical DNA molecules, each with one old and one new strand. In transcription a complementary mRNA is made in the nucleus on one strand of DNA as a template (uracil instead of thymine: A–U, T–A, G–C, C–G). The genetic code is the writing of 20 amino acids with 4 kinds of nucleotides: each amino acid is specified by three nucleotides in mRNA, a triplet (codon). There are 4 × 4 × 4 = 64 codons: 61 code for amino acids (many amino acids by several codons) and 3 (UAA, UAG, UGA) stop synthesis; AUG marks the start and codes for methionine. The code is universal: almost the same from bacteria to humans. In translation the ribosome moves along the mRNA, a tRNA with an anticodon complementary to each codon brings the matching amino acid, and the amino acids are joined by peptide bonds. A gene is a segment of DNA carrying information about one protein (or RNA). Short table: AUG – Met, UUU – Phe, GGC – Gly, GAA – Glu, UGG – Trp, AAA – Lys, CCU – Pro, GCU – Ala.

Worked examples
If the DNA strand is TAC–AAA–CCG, the mRNA is AUG–UUU–GGC and the protein Met–Phe–Gly.
The part coding for a 100-amino-acid protein has 100 × 3 = 300 nucleotides; at 0.34 nm per nucleotide the length is 300 × 0.34 = 102 nm.
Class activity

“Protein factory”. Four groups from the class: DNA (the code), mRNA (copier), ribosome (reader), tRNA (amino acid carrier). A game of “assembling” a protein from given DNA triplets; use the table.

Practice
1
If a DNA strand is TACGAATGG, write the mRNA.
2
If the DNA strand is TAC–CTT–ACC–ATT, find the mRNA and protein (from the table). ATT → UAA is a stop.
3
A protein has 150 amino acids. How many nucleotides are in the part of mRNA that codes for it (not counting the stop codon)?
4
Why is the universality of the genetic code important for biotechnology?