☰ Year plan · Biology

Why reproduce? Asexual reproduction

Week 6 · Chapter 3 Reproduction in Living Organisms (A): objectives, importance of reproduction, reproductive capacity, asexual reproduction: binary fission, budding, regeneration, spores (sporogony), parthenogenesis, tissue culture · 3 lessons
3

Eggs without fertilization and plants grown from tissue

Biology Sec 3 Term 1, Chapter 3: pp. 39–46
GoalExplain parthenogenesis in the honeybee and the aphid, and describe tissue culture and what it showed about plant cells.
New words
parthenogenesis · an egg develops into a new individual without being fertilized by a male gametehaploid · with one set of chromosomes, N; the number halved by meiosissomatic cell · an ordinary body cell, not a gametetissue culture · growing pieces of tissue or isolated cells in a nutrient medium until they form a whole organism
Explanation

Parthenogenesis is the ability of an egg to develop into a new individual without being fertilized by a male gamete. It is counted as a special kind of asexual reproduction because the offspring come from the mother only. It occurs in some worms, crustaceans and insects. In the honeybee the queen lays some eggs that develop without fertilization and become drones, which are haploid (N). Other eggs are fertilized and become diploid (2N) queens or workers, depending on the food the larvae are given. In the aphid, eggs made by mitosis develop without fertilization into diploid females, but when the aphid reproduces sexually its eggs are made by meiosis and are fertilized before they develop. Scientists have also started the development of eggs of the frog and the sea star without sperm, by exposing them to heat, an electric shock, radiation, certain salts, shaking or pricking, and early stages of rabbit embryos were obtained in a similar way. This is told here as the history of an idea; it is not something for a student to try. Tissue culture is carried out on plants. Tissues are grown in a semi-natural nutrient medium and followed until a whole organism develops. In a well-known experiment small pieces of carrot were placed in flasks of coconut milk, which supplies plant hormones and nutrients, and grew into whole carrot plants. Later, separated single cells of the same plant were grown in the same way and also gave whole plants, and a whole tobacco plant was obtained from isolated leaf cells. The experiments showed that every somatic cell of a plant carries all the genetic information, and that it can become a whole plant in a medium with plant hormones in the right ratio. The method is used to propagate rare plants, plants of a desirable kind and plants more resistant to disease.

AnimalEgg is made byIs it fertilized?Offspring
Honeybeethe queennodrones, haploid (N)
Honeybeethe queenyesqueens and workers, diploid (2N)
Aphidmitosisnodiploid (2N) females
Aphid (sexual reproduction)meiosisyesnew aphids from fertilized eggs
Worked examples
A hive with invented numbers. Suppose one worker has 32 chromosomes (2N = 32). A drone, developed from an unfertilized egg, is haploid, so it has 32 ÷ 2 = 16 chromosomes. If the queen lays 100 eggs and 30 are not fertilized, then 30 become drones and 100 − 30 = 70 are fertilized.
Growing a rare plant, with invented numbers. A nursery has one rare plant. A piece of it is placed in a nutrient medium and gives 12 new plants. If each of those 12 gives 12 more in the same way, there are 12 × 12 = 144 plants, all with the same genetic information as the first. This works because every somatic cell has the whole set of information.
Which result tells us what? A whole tobacco plant grew from isolated leaf cells. That tells us that a leaf cell, an ordinary somatic cell, still carries all the genetic information of the plant.
Class activity

Draw a hive family tree on paper, with a grown-up. Write queen at the top. Draw one line to a drone and label it unfertilized egg, N; draw another line to a worker and label it fertilized egg, 2N. Use the number 32 for the worker and work out the number for the drone. Then write two sentences explaining why a gardener can make many identical plants from one leaf in a laboratory but not by planting its seeds.

Practice
1
What is parthenogenesis?
2
In a honeybee colony, which individuals are haploid?
3
What did the carrot and tobacco experiments show about plant somatic cells?
4
Give one use of tissue culture.