Enzymes and recombinant DNA
Answers are for parents and teachers.
1
Write the function of: polymerase, ligase, restriction enzyme.
Polymerase – synthesises a DNA strand; ligase – joins fragments; restriction enzyme – cuts DNA at specific sites.
2
A circular plasmid has 2 EcoRI sites. How many fragments result after cutting? (Number only.)
2
3
Write the advantage of genetic engineering in making insulin.
Human insulin is obtained cheaply, in large amounts, pure, and without depending on animal organs.
4
What is gene cloning?
A foreign gene is put into cells with a vector, and its copies multiply as the cells divide.
5
If a bacterium divides every 30 minutes, how many cells does one give in 4 hours? (Number only.)
256